Request for Site directed mutagenesis Protocols (PCR)

kang at msvax.mssm.edu kang at msvax.mssm.edu
Fri Mar 3 01:07:10 EST 1995


In article <3j4n2f$6q6 at masala.cc.uh.edu>, benedik at uh.edu (benedik at uh.edu) writes:
>We are about to embark on making a number of site directed mutants and
>I greatly appreciate any comments people have on PCR methods to do
>this. Are there some protocols that reliably work for people which you
>can recommend.
>
>Thanks
>
>
>Michael Benedik               benedik at uh.edu
>Biochemical Sciences
>University of Houston
>Houston, TX 77204-5934


I used to use several methods. Among them Promega Altered mutagenesis system
was the best. (If it is wrong sorry for my ignorance. This is because I haven't
tried possibly better methods). 

I don't know the real best one buit I know the real worst one.
If you want to know send me e-mail.

Chulho Kang




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