Highly competent cells

[kang at msvax.mssm.edu]

In article <3i064d$es1 at unicorn.nott.ac.uk>, mbxrh at unicorn.ccc.nottingham.ac.uk (mbxrh) writes:
>I have a method for producing electrocompetent cells with a high
>efficiency.  I should note that i've not actually tried the method that
>was given to me yet.  I'm told that the efficiency of the cells
>decreases by several orders of magnitude if the cells are frozen.  Does
>anyone have a method of storing highly competent cells without the loss
>of efficiency?
>Any help would be greatly appreciated.
>Thanks in advance for any help.
>Richard Hastings (mbxrh at unicorn.nott.ac.uk).


If you just want to have high 10^7 or low 10^8 cell send me e-mail.
I have simple recipe.