SDS Removal/hemolytic protein

Karl Fischer kfischer at gpu.srv.ualberta.ca
Fri Mar 3 18:18:07 EST 1995


Jan,

Try soaking the gel overnight at 4°C with a couple of changes of 50 mM
Tris-HCl (pH 7.5). We use it to remove SDS (0.04%) from gels prior to in
situ testing of DNA polymerase/RT activity; this allows for renaturation
of the separated proteins.

Cheers

Karl

-- 
Karl Fischer
kfischer at gpu.srv.ualberta.ca
tyr-2 at bones.biochem.ualberta.ca



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