Jan, Try soaking the gel overnight at 4°C with a couple of changes of 50 mM Tris-HCl (pH 7.5). We use it to remove SDS (0.04%) from gels prior to in situ testing of DNA polymerase/RT activity; this allows for renaturation of the separated proteins. Cheers Karl -- Karl Fischer kfischer at gpu.srv.ualberta.ca tyr-2 at bones.biochem.ualberta.ca