houthaeve at embl-heidelberg.de
Fri Mar 3 07:27:05 EST 1995
> I'm working with a hemolytic protein and routinely use RBC
overlays of native gels to visualize activity. What I would like to
do is now run SDS polyacrylamide gels and overlay them with RBCs but I
need some advice on how
to get rid of the SDS. I tried washing one gel with a soln containing
casein hydrolysate (the original recipe called for casein) but saw
lysis in all protein areas then saw complete lysis throughout the
overlay. Any suggestions would be appreciated.
> Thanks in advance,
Why not blot your protein onto Nitrocellulose, wash and overlay with
RBC? Should be easier...
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