HELP: streaks in isozyme electrophoresis

Mikael Stenstrom mikael.stenstrom at systbot.gu.se
Fri Mar 3 08:14:00 EST 1995


I'm using electrophoresis on cellulose acetate to detect genetic
variation in Saxifraga oppositifolia, an arctic, perennial herb. 
I'm having problems with streaks that obscure some of the zones
with activity. I suppose this is due to phenolic compounds, but
using extraction buffers with, e. g., PVP, PVPP or BSA doesn't
help. Has anyone else had similar problems, and maybe even has
some clue on what to do?

Thanks in advance

Mikael Stenstrom
Dept. of Systematic botany
Univ. of Gothenburg, Sweden



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