EDTA in hybridization?
A. F. Cockburn
afc at nervm.nerdc.ufl.edu
Fri Mar 3 16:01:09 EST 1995
inkim at carina.unm.edu (In C. Kim) wrote:
> Hi all,
> I have a question on the role of EDTA for Southern/Northern
> hybridization. Some hybridization buffer contain EDTA, and some don't.
> For example, Church hybridization buffer includes 1 mM EDTA. My
> questions are:
> a) Is EDTA necessary for optimal hybridization?
> b) If so, what does EDTA do?
> --In C. Kim
b) It chelates divalent anions, which can cause nucleic acid
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