EDTA in hybridization?

A. F. Cockburn afc at nervm.nerdc.ufl.edu
Fri Mar 3 16:01:09 EST 1995


inkim at carina.unm.edu (In C. Kim) wrote:
>
> Hi all,
> 
> I have a question on the role of EDTA for Southern/Northern 
> hybridization.  Some hybridization buffer contain EDTA, and some don't.  
> For example, Church hybridization buffer includes 1 mM EDTA.  My 
> questions are:
> 
> 	a) Is EDTA necessary for optimal hybridization?
> 	b) If so, what does EDTA do?  
> 	
> Thanks.
> 
> --In C. Kim

a) No.
b) It chelates divalent anions, which can cause nucleic acid 
degradation.  

Andrew Cockburn



More information about the Methods mailing list