Is it Taq or the primer responsible for the mistake?
landel at helios
Fri Mar 3 15:47:37 EST 1995
In article <01HNP6F12GKI00PF24 at post-office.uh.edu>,
carlos h. pedemonte <CPedemonte at UH.EDU> wrote:
>We have sequenced part of a plasmid and found a missing base. Since we
>have used PCR with Taq polymerase to produce this part of the plasmid, we
>want to identify what have caused this deletion. We think that the problem
>is in the custom-made primer. But we would like to hear your opinion, to
>be sure that our conclusion is correct.
We had this problem once. We solved it by getting new primer from a
different company. We also sent a nastygram to our original supplier
and have never bought from them again.
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