RNA from Liver

[Karl Fischer]

In article <saleem.14.001DB4F7 at rfhsm.ac.uk>, saleem at rfhsm.ac.uk (Dr Saleem
Mohammed) wrote:

> I have been isolating total RNA from Rat liver, and have had a few problems 
> with purity and integrity.
> 
> Originally I was using RNAzol B (Biogenesis Ltd, Single step Method) which 
> gave me RNA containing lots of protein which had not been removed.  I tried 
> many things like extra phenol:chloroform extractions and reducing the amount 
> of starting liver etc but no real luck.  The RNA was still contaminated with 
> protein.

If it is protein and not glycogen (as has happened to us before) you might
want to digest your aqueous phase with proteinase K for a couple of hours
followed by phenol:chloroform extraction.

Karl

-- 
Karl Fischer
kfischer at gpu.srv.ualberta.ca
tyr-2 at bones.biochem.ualberta.ca