How do I make a TA cloning vector?
hodges bradley l
segdoh at uxa.cso.uiuc.edu
Fri Mar 3 13:50:05 EST 1995
Holton, T.A., Graham, M.W., A simple and efficient method for direct
cloning of PCR products using ddT-tailed vectors. Nucleic Acid Research,
Vol. 19, N0.5 pp. 1156, 1990
On 2 Mar 1995, Markus Grompe, MD wrote:
> I am looking for a protocol how to make myself a nice batch of vector
> with a single T overhang for cloning PCR products.
> Does anyone out there have a good protocol for home use?
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