How do I make a TA cloning vector?

hodges bradley l segdoh at
Fri Mar 3 13:50:05 EST 1995

Holton, T.A., Graham, M.W., A simple and efficient method for direct 
cloning of PCR products using ddT-tailed vectors.  Nucleic Acid Research, 
Vol. 19, N0.5 pp. 1156, 1990

On 2 Mar 1995, Markus Grompe, MD wrote:

> I am looking for a protocol how to make myself a nice batch of vector
> with a single T overhang for cloning PCR products.
> Does anyone out there have a good protocol for home use?

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