Okazaki fragments -- in Thermicus aquaticus?
rb690253 at bcm.tmc.edu
Fri Mar 3 20:58:41 EST 1995
In article <3ivuf2$kqu at elaine30.Stanford.EDU>, ladasky at leland.Stanford.EDU
(John Ladasky) wrote:
> Greetings, everyone,
> I was just wondering. Since Thermicus aquaticus (the bacterium
> that gave us Taq polymerase) lives at 72 degrees C, how does it prime its
> DNA replication? It seems to me that the synthesis of an Okazaki fragment
> on the lagging strand would somehow have to be "protected" in order that
> it remains annealed. Any thoughts? Has any reseach been done?
> By the way, if someone can think of a better newsgroup in which
> to discuss this issue, we can move.
> Unique ID : Ladasky, John Joseph Jr.
> Title : BA Biochemistry, U.C. Berkeley, 1989
> Location : Stanford University, Dept. of Structural Biology, Fairchild D-105
> Keywords : immunology, music, running, Green
I don't know if any research has been done, but it is an interesting
question. Do we even know that this bugs DNA is replicated semi
discontinuously? Probably so. I would assume that there is a primase
that initiates Okazaki fragment synthesis but what holds the fragment in
place until the fragments are ligated together I'm not sure. Perhaps a
high GC content? Or is there a specific mechanism? It would sure be
interesting to find this factor(s), not to mention potentially very
helpful to the PCR reaction.
Baylor College of Medicine
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