heteroplasmy of cloning vectors
G
G
Fri Mar 3 10:17:39 EST 1995
This may be a stupid question but it occured to me this week whilst I
was trying to clone a PCR product. Is it possible for transfomed cells
to be heteroplasmic, i.e. to contain different species of plasmid perhaps
one type with an insert and one without? I want to clone a PCR product to
use as an internal standard, if heteroplasmy occurs then my calculated
copy number will be elevated due to the possible presence of plasmids with
no insert.
G Jenkins
More information about the Methods
mailing list