Pulse field gels

biukovic at olimp.irb.hr biukovic at olimp.irb.hr
Fri Mar 3 17:54:38 EST 1995


In article <3j1dqu$3t1 at mserv1.dl.ac.uk>, "MS WF HONG [GEN]53872" <GEN282E at ccs1.cc.monash.edu.au> writes:
> Dear netters,
>       I am working on genomic mapping of Pseudomonas by pulse field 
> gel electrophoresis.  At this stage, I have a problem in separating a 
> 173kb doublet.  Does any one have suggestions on how to solve this 
> problem.
> 
> 
>        Thanks in advance.
>        
>        
>        
> W. F. Hong       
> 

Well, the best way probably is making a gene library and pulling out
linking clones  hybridizing the library with the 173kb doublet
probe.Making the library shouldn't be problem considering the high
and reliable packaging efficiency of commercial pack. mix (Stratagene)
like Gigapack
Good luck. Goran Biukovic , biukovic at olimp.irb.hr 



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