In vitro transcription ......
Martin D. Leach
leach at bu.edu
Fri Mar 3 11:01:19 EST 1995
In article <3j49va$hro at netnews.ntu.edu.tw>, r3211001 at cc.ntu.edu.tw
> Dear netter,
> I want to find out the cis-regulatory elements of an oocyte-specifically
> expressed gene. Because of some technique problems, I cannot transfect the
> reporter constructs into the living cells. My advisor told me to use 'in
> vitro transcription assay' in cell extracts. I've tried several times,
> but it did not work.
How about injecting supercoiled reporter constructs into the
oocytes...e.g. lacZ and assaying them...
alternatively...use a luciferase construct...and see if the oocytes glow
in the dark (scintillation counter with attached single photon monitor
would be a good choice for using a whole vial of oocytes) upon addition of
Lucifierin (K+ salt) - 5mM ? correct me.
-BTW I am not only the luciferase president...i am also a client..-
..... Martin Leach Email:leach at bu.edu
_|____ Dept. of Pharmacology Phone: (617) 638-5323
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