EtBr in agarose gels...

Ian Lyons ilyons at
Sat Mar 4 23:40:13 EST 1995

If you want to quantitate the amount of DNA in a band on a gel it's
important to incorporate the EtBr into the gel (at 0.5ug/ml).  
This gives much better linearity between fluorescence and DNA amount.
Try it with markers if not convinced.  
ian lyons (ilyons at

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