Question: PCR With 500bp Primer?

Steven Sullivan sullivan at
Sun Mar 5 17:29:33 EST 1995

Yi, Xiaoming (Z3C20 at wrote:
: Hi, netters,

: I have posted this question a couple of days ago, but got not response. Did
: anyone do PCR with a big primer like 500bp? If so, can anybody tell me what the
: major difference is, what should I do to make it work?

: Thanks!

A 500bp primer would cost a lot more than a 20-mer . ;>

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