EtBr in agarose gels...

Shahram Mori smori at nmsu.edu
Mon Mar 6 01:23:25 EST 1995


KirstyTownsend wrote:

: In article <Pine.A32.3.91.950222182822.74692A-100000 at rs5.tcs.> Brick Ayola <abrick at mailhost.tcs.tulane.edu> writes:
: >
: >Regarding EtBr staining of nucleic acids:
: >Can one simply add EtBr to the loading dye (as with some RNA gel 
: >protocols)? Or will this significantly disturb the migration of DNA 
: >super-coils and such?
: >
: >Brick Ayola, Ph.D.
: >Tulane School of Medicine

I have noticed that if you are running Polyacrylamide gels and using small
fragments that it is best not to add the EtBR to your loading sample. The DNA
runs really funny.
Cheers
--
Shahram Mori					   _/\_
Program in Molecular Biology			  _\  /_
Dept. of chemistry and Biochemistry Box 3C	  \_  _/
NMSU  Las Cruces NM				    ||
88003





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