EtBr in agarose gels...
rapr at med.pitt.edu
Sun Mar 5 15:09:29 EST 1995
In article <3jbfbe$fih at quandong.itd.adelaide.edu.au>, Ian Lyons
<ilyons at biochem.adelaide.edu.au> wrote:
> If you want to quantitate the amount of DNA in a band on a gel it's
> important to incorporate the EtBr into the gel (at 0.5ug/ml).
> This gives much better linearity between fluorescence and DNA amount.
> Try it with markers if not convinced.
This is difficult to believe; it suggests that the gel without EtBr in it
was simply not stained long enough, or that the conc. of EtBr in the
staining solution was in error. Can you cite details or a published ref.
for your assertion? Thanks.
Robert Preston rapr at med.pitt.edu
Highland Park 40deg28'N 79deg55'W Pittsburgh PA 15206
U. Pittsburgh Sch. Med. S792 Scaife Hall Pittsburgh PA 15261
[vox 412-648-9573 fax 412-648-1916 (W)] vox 412-361-8905 (H)
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