Genomic DNA from cells (re: Kits)
Dr Keith Anthony Grimaldi
regokag at ucl.ac.uk
Sun Mar 5 14:11:15 EST 1995
Recently somebody was asking about kits for isolating genomic DNA from
mammalian tissue. In my lab we have recently developed a very simple, quick,
'single-tube' method for isolating DNA. It can handle up to 3-5 million cells
in a single eppendorf tube. The DNA isolated is of high MW, and has been use
for restriction digestion, quantitative and ordinary PCR, and Southern
blotting. It is simple and most of all v.v. cheap.Its submitted to
Biotechniques, but if anyone is interested I can Email the text. My address is
k.grimaldi at ucl.ac.uk.
--
Keith Grimaldi
Dept. Oncology
University College London TEL: (0)171-636-8333 ext:3419
91, Ridinghouse Street
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