kitchingman at kitchingman at
Sat Mar 4 09:22:38 EST 1995

In article <kgraham-0203951812510001 at>, kgraham at (kevin c. graham) writes:
> Hi there, this is a first-time post so please bear with me.
> A while ago I read on this group that someone was using a combination of
> ampicillin and methicillin in their LB plates to select for E. coli
> transformants. This was a cheaper alternative than using carbenicillin.
> I've ordered the methicillin but can't remember what concentrations of
> each to use!  
> Could someone please help? Thanks.
80 micrograms per ml of methicillin and 20 micrograms per ml of ampicillin. 
Works great, no satellite colonies unless you incubate your plates for over 24

Geoff Kitchingman
kitchingman at

"Perception is everything, reality is irrelevant"- American political saying

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