methicillin&ampicillinREGISTER

kitchingman at mbcf.stjude.org kitchingman at mbcf.stjude.org
Sat Mar 4 09:22:38 EST 1995


In article <kgraham-0203951812510001 at gietz.hgen.umanitoba.ca>, kgraham at bldghsc.lan1.umanitoba.ca (kevin c. graham) writes:
> Hi there, this is a first-time post so please bear with me.
> 
> A while ago I read on this group that someone was using a combination of
> ampicillin and methicillin in their LB plates to select for E. coli
> transformants. This was a cheaper alternative than using carbenicillin.
> I've ordered the methicillin but can't remember what concentrations of
> each to use!  
> Could someone please help? Thanks.
> 
80 micrograms per ml of methicillin and 20 micrograms per ml of ampicillin. 
Works great, no satellite colonies unless you incubate your plates for over 24
hours.

Geoff Kitchingman
kitchingman at mbcf.stjude.org

"Perception is everything, reality is irrelevant"- American political saying




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