DNA prep for transient transfection

merle
Mon Mar 6 16:13:02 EST 1995


> In Article <nick.5.2F559DCF at lmb1.rug.ac.be>
> nick at lmb1.rug.ac.be writes:

> >
> >As a coincidence, we had a discussion only yesterday about problems with low 
> >efficiencies of transient transfections in our lab.
> >
> >More precisely, transfections using the DEAE-dextrane method seem to
give very 
> >variable results, given one plasmid preparation to another.
> >
> >The low efficiency is independent of the person, the acceptor cells or
the DNA 
> >itself. In fact...the problem seems to be the preparation method used. Sorry 
> >to tell you so, but this all began when we started using QIAGEN columns!


the protocol we follow for our DNA preps is long but we get a good yield
of very clean DNA and have no problems with transfections 
if you are interested i can send you the protocol .. but we do not use a
column of any sort



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