PCR using lambda gt11 primers

elida at parasit.lan.mcgill.ca elida at parasit.lan.mcgill.ca
Mon Mar 6 17:26:30 EST 1995


I have purified clones from a lambda gt11 library and I am having problems when trying removing the insert from the EcoR1 
cloning site (EcoR1 does not cut the purified DNA, while other restriction enzymes do). I would like to know if anyone in this 
net has ever tried to use PCR to amplify inserts in lambda gt11 using specific lambda gt11 primers. I have tried without 
success. 
If you know the secret, please help me!!!
Elida.





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