DNA prep for transient transfection
Ian A. York
york at mbcrr.dfci.harvard.edu
Mon Mar 6 13:02:09 EST 1995
In article <3jfav3$n2e at amcnix.amc.uva.nl> seppen at amc.uva.nl writes:
>we had a similar problem with obtaining stable transfectants. I now routinely
>do one phenol and two chloroform/iaa exctractions after the maxiprep with
>qiagen. The interfase always contains protein, so qiagen is probably not
>enough to get pure vector.
Hmmm. This is important. I have seen large variations in
efficiency of transients, but I can't say that they were or were not
because of the Qiagen preps. I'll try to set up side-by-side assays and
will report back.
Has anyone else done this, or have any comments?
Ian York (york at mbcrr.harvard.edu)
Dana-Farber Cancer Institute, 44 Binney St., Boston MA 02115
Phone (617)-632-4328 Fax (617)-632-2627
More information about the Methods