TRANSFER OF PLASMIDS INTO BACTERIA

[BIO101 at DELPHI.COM]

betts at ORION.ETSU.EDU (GORDON BETTS) writes:

all
 





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>I have obtained a pair of plasmids (a whole 1 ul of each) and now must do
>something with them.  Since I am new to this area of work, I'm hesitant to
>just jump in.  So, does anyone have good advice on how to do a one-time,
>not-a-chance-of-failure transfection into bacteria?  What's a good vector
>for this?
>Thanks,
>Gordon Betts
>Biology Dept.
>East Texas State University
>Commerce, TX 75429-3011
>903/886-5369
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>Life is like a bowl of stew, you need to stir things up once in a while so 
>the scum doesn't rise to the top.
>      Author unknown
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