random hexamers as primers for RT in RT-PCR

Sean David Moore smoore at mail1.sas.upenn.edu
Tue Mar 7 11:45:19 EST 1995


I don't think the molecules would "join up" as you suggest without help.  
Also your assumption about the distance is mostlt right.  You must also 
consider the length of the poly-A, and if it is present atall in some 
cases.  there could also be some sort of strong stop in the sequence 
before the target region, Random Hexies are a good way around most of 
that, but remember not to go cDNA cloning from the products.


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