Disposable filters for buffers etc
aquilla at salus.med.uvm.edu
Tue Mar 7 10:44:49 EST 1995
In Article <newitt-0303951116510001 at 188.8.131.52>, newitt at ncifcrf.gov
(John A. Newitt) wrote:
>In article <3j5nt4$imn at usenet.INS.CWRU.Edu>, bl275 at cleveland.Freenet.Edu
>(Dan Diaz) wrote:
>> ive been unhappy with the performance of nalgene's disposable filterware,
>> which i use for filter sterilization and for particle removal from hplc and
>> fplc buffers.
>> more often than not, i find little bits of material in the filtrate which
>> apparently come from the filter itself.
>I've not had any problems with Nalgene nor Corning disposable filterware
>when filtering aqueous buffers. Now if you are filtering organic
>solvents, e.g. acetonitrile or concentrated methanol, that's another
>problem. Make sure that the chemical resistance of the filter and housing
>materials are suitable for the solutions you are filtering. Nalgene and
>many other manufacturers include this info in tables in their catalogs
>that contain filterware.
>John A. Newitt <newitt at ncifcrf.gov>
>National Institutes of Health
>Bethesda, Maryland USA
I've used these products for years with no problems. Are you filtering into
glassware? If so, this may be the source of the particulate matter. If you
store the filtrate in the disposable plastic container (i.e. more expensive
filter apparatus), you probably won't have a problem.
More information about the Methods