Ligation of alkaline phosphatase treated DNA

Martin Kennedy mkennedy at chmeds.ac.nz
Tue Mar 7 17:09:11 EST 1995


In article <3ir2ka$mep at nz12.rz.uni-karlsruhe.de>, uc05 at rzstud1.rz.uni-karlsruhe.de (Dietmar Baas) writes:
> I have a theoretical problem with the ligation of DNA, treated with
> alkaline phosphatase (a.p.) and here is my question:
 stuff deleted.........
> Another possibility is the "in vivo repair" of the gap after transformation
> by enzymes of E.coli (but this sounds like a strange theory).
>       Dietmar

Yes, this is the correct answer. The bacterial DNA repair 
machinary repairs the ss nick, just as it does during the
normal processes of DNA replication, recombintion and repair.
   
-- 
Cheers,

Martin

NNNN   NN  Martin A Kennedy (E-mail = mkennedy at chmeds.ac.nz)  ZZZZZZZ  
NN NN  NN       Cytogenetic and Molecular Oncology Unit          ZZZ
NN  NN NN           Christchurch School of Medicine            ZZZ
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