control for TPA induction

Manuel Maria SIMON Simon at mail.boku.ac.at
Tue Mar 7 12:52:29 EST 1995


lyc at IBMS.SINICA.EDU.TW ("L.Y.Chau") wrote:
>
> Dear netters:
>     Do you know which DNA probe can be used as the internal control for TPA 
> induction experiment.  I tried GAPDH but TPA seems to down-regulate the 
> level of GAPDH RNA.
> 
> Jonghwei

High Jonghwei

You can take a interleukin 1 probe. 
I do not know any cell type which does not turn on IL-1 upon
phorbol esters.

Good luck
Manuel





More information about the Methods mailing list