Replica plating of cells

Peter J. Hornsby phornsby at bcm.tmc.edu
Tue Mar 7 07:54:39 EST 1995


paiks at gunet.georgetown.edu states:

>I am using promoter trap to clone hormone regulated genes from breast cancer
>cells. Since the frequency of the event that I am looking for is very low, I
>am interested in developing replica plating method for anchorage dependent
>breast cancer cell lines. I have tried the nylon mesh with glass beads
>several times in vain. I would greatly appreciate if anyone can provide me
>with working protocol.

Please see our modification of the replica plating protocol published in
BioTechniques 12: 244 (1992). If you need any specific help, please E-Mail
me.

Peter Hornsby, Baylor College of Medicine




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