Okazaki fragments -- in Thermicus aquaticus?

Duncan Clark Duncan at genesys.demon.co.uk
Tue Mar 7 10:50:47 EST 1995


> 
>         I was just wondering.  Since Thermicus aquaticus (the bacterium 
> that gave us Taq polymerase) lives at 72 degrees C, how does it prime its
> DNA replication?  It seems to me that the synthesis of an Okazaki fragment
> on the lagging strand would somehow have to be "protected" in order that
> it remains annealed.  Any thoughts?  Has any reseach been done? 

If you think it is difficult at 72C what about the hyperthermophilic 
that grow above 100C ie Pyrodictium, Hyperthermus etc

Duncan 


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