Tm of a DNA hairpin

HARDIES at THORIN.UTHSCSA.EDU HARDIES at THORIN.UTHSCSA.EDU
Tue Mar 7 10:52:49 EST 1995


Alain Laigle writes:

> Could somebody tell me whether the Tm of a hairpin in a single stranded DNA
depends on the salt concentration.

It should, but I wouldn't count on it following the same mathematical form
as for the intermolecular reaction.  Does your question arise from a 
PCR primer problem?

Steve Hardies, Assoc. Prof. of Biochem., Univ. of Texas HSC at San Antonio
Hardies at uthscsa.edu



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