Definition: Saturation Mutagenesis
rapulak at macc.wisc.edu
Tue Mar 7 12:42:03 EST 1995
In article <anderson-060395172733 at 220.127.116.11> Eric C. Anderson,
anderson at pharmdec.wustl.edu writes:
>so far, all i've been able to find in the way of a definition or
>description came from Lewin's Geves IV where they consider it the
>of every nucleotide in a particular region. my personal vote would be
>mutating every amino acid to something different, preferably not a
If your gene was a tRNA gene, then how would you define saturation
mutagenesis? Do you think the term should have gene specific meanings?
(IMHO) I don't think it should.
Also, it may just be a poor term in general. Should saturation
mutagenesis be every nucleotide for every other nucleotide, one
at a time, then two at a time, then three at a time, and so forth?
Real "saturation" of all possible mutations would be just that.
Some of forms of SELEX achieve this. Most site directed mutagenesis
experiments fall far short of these goals.
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