Q:Does Gene Clean interfere with ligation?

Simon Dawson mbxspd at unicorn.nott.ac.uk
Wed Mar 8 03:56:19 EST 1995


Hi,
   'new wash' is almost bound to inhibit ligations if its there in any
quantity as its 70%(?) ethanol. I spin the glass and remove most of the
wash respin and remove as much of the residual wash as I can with a
fine tip. Leave the pellet to air dry on the bence for maybe 5-10
mins (vacuum drying decreases the yield a lot) and elute with TE.
  Typically, yields are >= 80%.
  Hope this helps,

         Simon.


Simon Dawson
Dept. Biochemistry
QMC
Nottingham
U.K.

Email: mbxspd at unicorn.nott.ac.uk
URL: http://128.243.40.7/~mbxspd/Simon.html



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