Q:Does Gene Clean interfere with ligation?
mbxspd at unicorn.nott.ac.uk
Wed Mar 8 03:56:19 EST 1995
'new wash' is almost bound to inhibit ligations if its there in any
quantity as its 70%(?) ethanol. I spin the glass and remove most of the
wash respin and remove as much of the residual wash as I can with a
fine tip. Leave the pellet to air dry on the bence for maybe 5-10
mins (vacuum drying decreases the yield a lot) and elute with TE.
Typically, yields are >= 80%.
Hope this helps,
Email: mbxspd at unicorn.nott.ac.uk
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