la pcr complexes

aEscher aEscher
Wed Mar 8 20:16:27 EST 1995


We are amplifying a 6 kb fragment from mouse genomic DNA. No problem, but
suddenly from one day to the next all we can see is a high MW complex 
stuck in the well. This phenomenon seems to be the result of the amplifi-
cation reaction. Does anyone know what's happening? 
Thanks,
Alan Escher.
                             




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