GFP in CHO cells

ICAgen ICAgen at
Wed Mar 8 21:39:45 EST 1995

To reply to a prior post:  I've had poor efficiencies (~5%) when 
transfecting CHO cells with a GFP construct in pcDNA3 using the calcium 
phosphate method.  Using lipofectamine boosts the efficiency to around 
40% or more, so it's not (n=2) a problem with the cells, but rather with 
the transfection method used.


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