ayele_bandele at qmcumc.mail.cornell.edu
Wed Mar 8 16:09:29 EST 1995
> This is my first time posting so bear with me.
> I recently started running 2-D gels using the Bio-Rad system.
> My first question is what can be done to prevent the horizon-
> tal lines in the gel?
> I am solubilizing in 2% NP-40,4% ampholyte, 9.5M Urea and 100mM DTT.
> Next question, when running an 8mm X 10mm 2nd dimension gel, is it
> normal for it to run for an hour > and 45 minutes.
> Finally, I recently purchased gelcode color Silver Stain; but the
> only colors I see are brown and yellow spots on an amber background. Normal?
> Does this group have a FAQ listing? if so how do I find it?
> Hope someone can help me :->
More information about the Methods