Transfecting Primary Cell Lines

[Jan Eggermont]

In article <dcox.2.000C374A at medmicro.uct.ac.za>, dcox at medmicro.uct.ac.za
(Dermot Cox) wrote:

> We are at present having problems transfecting primary cells derived from
> foetal bovine testes with plasmid DNA.  We are using the DOTAP kit from
> Boehringer-Mannheim with little success.  Has anyone had similar problems with
> this kit or could make suggestions of alternate methods.
>
A while ago I tested the transfection efficiency of DOTAP versus
lipofectamine (GibcoBRL) on an established endothelial cell line. My
impression was that lipofectamine was vastly superior.

Regards,

Jan

--
Jan Eggermont                       Jan.Eggermont at med.kuleuven.ac.be
Laboratorium voor Fysiologie - KU Leuven
Campus Gasthuisberg
B-3000 Leuven                            tel 32-16-345938
Belgium                                  fax 32-16-345991