Re-using hybridisation solutions containing double-stranded probes?
Jan.Eggermont at med.kuleuven.ac.be
Wed Mar 8 07:18:20 EST 1995
In article <01HNG6291XDUA5U66B at MPGARS.DESY.DE>, DREWES at MPASMB.DESY.DE wrote:
> Has anybody ever tried re-using plaque hybridisation solutions containing
> 32P-labeled double stranded probes? It would mean a significant reduce in
> radioactive waste and expensive stuff like salmon sperm DNA and deionized
> formamide. I am thinking about simply storing the used solution in the freezer
> and then denature the DNA and the probe again by boiling for ten minutes
> the next use.
Yes, you can reuse hybridisation solutions. After hybridisation we collect
the hybridisation mix and store it at -20 C. Prior to a new
hybridisation, we heat it up to 65 C for 15 min prior. I think boiling the
mix before usage may cause precipitation of BSA in the mix...
Jan Eggermont Jan.Eggermont at med.kuleuven.ac.be
Laboratorium voor Fysiologie - KU Leuven
B-3000 Leuven tel 32-16-345938
Belgium fax 32-16-345991
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