Preparartion of Liver DNA for PFGE
Dr Saleem Mohammed
saleem at rfhsm.ac.uk
Wed Mar 8 23:10:22 EST 1995
I was wondering if anybody had a method for analysing rat liver genomic DNA
by pulse field gel electrophoresis. I have talked to a number of people who
suggest using agarose plugs.
The question is how does one break liver cells from frozen liver (liquid
nitrogen). Could one just homogenise using a pestle and mortar and then
prepare agarose plugs from the powdered liver and if so how much liver to use
The alternative is to isolate genomic DNA using traditional
proteinase K, RNAase and phenol/chloroform extractions, although this can
damage genomic DNA.
ANY IDEAS would be greatly appreciated.
Dr Saleem Mohammed,
Dept of Biochemistry & Molecular Biology,
Royal Free Hospital School of Medicine.
Rowland Hill Street,
London. NW2 2PF.
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