Enhancement of PCR?

Zal Suldan Z-Suldan at ski.mskcc.org
Thu Mar 9 08:53:51 EST 1995


> There were some posts a few weeks ago regarding deterioration of primers. 
> Appearently some primers fail to amplify after storage in liquid at 
> either 4 oC or -20 oC. It was suggested that the primer form 
> intramolecular bonds which limit its effectiveness. The boil/freeze helps 
> to restore activity. 

I'm confused.... aren't we about to add these very same primers to a
PCR reaction which will heat them, again, to 95C and then cool them,
somewhat quickly, into the 40's or 50's. Wouldn't that make the snap
freezing moot? Just curious.

Zal



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