Enhancement of PCR?
Z-Suldan at ski.mskcc.org
Thu Mar 9 08:53:51 EST 1995
> There were some posts a few weeks ago regarding deterioration of primers.
> Appearently some primers fail to amplify after storage in liquid at
> either 4 oC or -20 oC. It was suggested that the primer form
> intramolecular bonds which limit its effectiveness. The boil/freeze helps
> to restore activity.
I'm confused.... aren't we about to add these very same primers to a
PCR reaction which will heat them, again, to 95C and then cool them,
somewhat quickly, into the 40's or 50's. Wouldn't that make the snap
freezing moot? Just curious.
More information about the Methods