How "random" are random primers?
mdcabl at cc.newcastle.edu.au
mdcabl at cc.newcastle.edu.au
Thu Mar 9 07:37:14 EST 1995
Hi all,
A friend of mine asked an interesting question. What is the G-C content of a
random hexamer? Before you flame, I know that it would be 50% if it is
truly random sequence. The more relevant question is perhaps is a random
hexamer truly random or is the G-C content decreased to increase the annealing
temperature of the hexamer. My friend had no luck with the local tech-reps.
Can someone out there help my friend out?
Cheers,
Allen Black
Dept. of Pathology
Univ. of Newcastle
mdcabl at cc.newcastle.edu.au
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