DMSO in PCR rxns ??

Glibert Fabrice glibert at ceph.cephb.fr
Thu Mar 9 06:39:04 EST 1995


In article <3jlmdi$6qs at server.st.usm.edu>, Glen Shearer <gshearer at whale.st.usm.edu> writes:
|> I think I saw a recent post about adding DMSO to PCR reactions
|> which gave better results.  True?  Or am I just dreaming this?
|> 
|> If so, what does the DMSO do?
|> 
|> Glen
|> [gshearer at whale.st.usm.edu]
|> 

i use Xylene Cyanol Blue in my PCR reaction for loading
my gel just after the PCR and i add in the reaction
sucrose
During my test i saw that the sucrose reduce the non
specifics bands perhaps because sucrose is a very large
molecule
i used it a 2 4 or 6% sucrose in final reaction
i hope that you understand what i mean because i know
i have a very bad english...sorry !

fabrice



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