DMSO in PCR rxns ??

Matthias Zeiner mzei at sun0.urz.uni-heidelberg.de
Thu Mar 9 06:36:16 EST 1995


On 9 Mar 1995 01:42:10 GMT, Glen Shearer wrote:

>I think I saw a recent post about adding DMSO to PCR reactions
>which gave better results.  True?  Or am I just dreaming this?

This is, in my experience, especially true for GC-rich templates, ie >75% 
GC. DMSO seems to lower the Tm of DNA, but this also means that one might 
have to adjust the annealing temperature. Also, specificity of the PCR can 
be enhanced, for obvious reasons.

Matthias Zeiner



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