Church for Northern blots?

Glen Shearer gshearer at whale.st.usm.edu
Fri Mar 10 14:03:04 EST 1995


We use a modified Church...  hyb soln (0.5 M NaPO4, pH 7, 5% w/v
SDS) for northerns.  Works like a charm!

By the way, use the cheap semi-pure SDS from Sigma instead of
the expensive molec. biol. grade.

Regards,
Glen
[gshearer at whale.st.usm.edu]






More information about the Methods mailing list