transient transfection

Emmanuel Skoufos, Ph.D. skoufos at nefeli.imbb.forth.gr
Fri Mar 10 12:36:08 EST 1995


In article <9503092222.AA25622 at uranus.med.pitt.edu>, corey at MED.PITT.EDU (Seth Corey) writes:
>
>we would like to do transient transfections 
>but COS cells express the gene of interest.
>any other good lines to use?
>thanks,
>seth

This is hard to answer unless you lets us know:
1) The gene you want to express in the cells.
2) A little more about the type of the expreriment (e.g. is there a reporter
involved)
3) Are there any tissue origin considerations.

Cheers

Emmanuel


_______________________________________________________________________________

Emmanuel Skoufos, Ph.D.
Insect Molecular Biology Group
Institute of Molecular Biology and Biotechnology
Box 1527 
Heraklion, Crete 71110
Greece



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