Does Klenow proofreading multiple-base 3' overhangs or just single base?

Fri Mar 10 11:53:26 EST 1995

Can anyone tell me if the Klenow proofreading activity used for trimming off 
the extra 3' nucleotide added during PCR RXNs also works for trimming back
multi-base 3' overhangs of restriction-digested fragment ends?  I've come 
across a number of papers which state in their M&M that a digested fragment 
was "blunted" with Klenow.  Since you can't "fill-in" in front of the 5' 
recessed end, they must mean the use of Klenow to nibble back.
Regards, Peter 
*  Peter M. Muriana, Ph.D.             317-494-8284   TEL            *
*  Dept. of Food Science               317-494-7953   FAX            *
*  Purdue University                   murianap at   *
*  Smith Hall                                                        *
*  W. Lafayette, IN  47907-1160                                      *

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