MeOH in Western Buffer
lhom at OCF.Berkeley.EDU
Fri Mar 10 13:03:30 EST 1995
My labmates are invariably astonished to hear that I don't put methanol in
my Western transfer buffer. But I never have, and I still get nice transfer.
Why do people put MeOH in their buffer? Does its absence not affect me because
of the membrane I use (nitrocellulose)?
Lou Hom >K'93 "It has been suggested that...gene splicing and cloning
lhom at ocf.berkeley.edu are so simple that one can successfully carry them out
in [any] modern kitchen. In spite of my considerable
Quote by respect for the potential of food processors and radar
B.R. Levin ranges, I very much doubt this to be the case."
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