How many DNA binds to Nylon membranes

Dietmar Baas uc05 at rzstud1.rz.uni-karlsruhe.de
Wed Mar 8 12:22:12 EST 1995


Hi,

I'm new on genomic screening and want to know the amount of DNA which binds
on a nylon membrane by simple plaque blot (all people say they can detect
1 or less pg of DNA, but nobody mentioned the amount of DNA usually bounded
on a membrane).
Are there great diffrences between uncharged and charged membranes (beyond
the price)?

Thanks in advance, Dietmar




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