Inverse PCR on cDNA

Gordon Stott gs2 at mole.bio.cam.ac.uk
Fri Mar 10 06:56:05 EST 1995


Has anybody got a tried and tested protocol for iPCR on cDNA as I'm trying
to clone out the unidentified 5' flank of a fusion transcript. it appears
to be present at low levels and has resisted all attempts at RACE and
homopolymeric tailing so far.

Thanks in advance for any suggestions,

Gordon



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