DMSO in PCR rxns ??
li_je at a1.mscf.upenn.edu
Fri Mar 10 18:15:57 EST 1995
In article <3jmp7g$35k at mserv1.dl.ac.uk>, "Matthias Zeiner"
<mzei at sun0.urz.uni-heidelberg.de> wrote:
> This is, in my experience, especially true for GC-rich templates, ie >75%
> GC. DMSO seems to lower the Tm of DNA, but this also means that one might
> have to adjust the annealing temperature. Also, specificity of the PCR can
> be enhanced, for obvious reasons.
> Matthias Zeiner
Please explain further the obvious reasons. They are still quite obscure
to me. Sorry!
Jie li U of PENN
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