Fluoro-dATP sequencing kit problem

Casey Chin cchin at crl.com
Fri Mar 10 16:54:45 EST 1995


I have found that I have had the best results using that Fluoro-dATP 
works best when the following conditions have been met:
	1. Somewhere in the next 8-10 bases there is one (and preferably 
	   only one) incorporation site is present.
	2. The primer that is used does not form a primer dimer.
	3. The primer is allowed to anneal specifically to a single 
	   site.



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